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KMID : 0361020070500050416
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Korean Journal of Otolaryngology - Head and Neck Surgery
2007 Volume.50 No. 5 p.416 ~ p.419
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Immunologic Response of Upper and Lower Airway Disease Patients Against Airborne Fungal Cultural Extracts
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Shin Seung-Heon
Kim Ki-Yeong
Ye Mi-Kyung
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Abstract
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Background & Objectives: Allergic rhinitis, sinusitis and asthma are linked by epidemiologic, physiologic, and immunopathologic characteristics and by similar therapeutic approach. They have clinical manifestations of a systemic inflammatory process within the respiratory tract. The purpose of this study was to determine the immunologic response of upper and lower airway disease patient’s peripheral blood mononuclear cells (PBMC) against airborne fungi.
Subjects & Methods: PBMC from ten healthy human volunteers (NC), fourteen chronic rhinosinusitis (CRS), eleven allergic rhinitis (AR), twelve allergic asthma (AA), and six non-allergic asthma (NAA) were stimulated with 50 microgram/ml Alternaria, Aspergillus, Candida, Cladosporium, and Penicillium. To determine the activation of PBMC, IL-5, IL-13, and INF-gamma were measured with the ELISA method.
Results: CRS, AR, AA patients produced large amounts of IL-5 (by Alternaria, Aspergillus, Cladosporium, and Candida) and IL-13 (by Alternaria) than NC. NAA and CRS patients produced larger amounts of INF-gamma (by Alternaria) than NC. Alternaria enhanced the production of cytokines most strongly, and Penicillium didn’t influence the production of cytokines.
Conclusion: Fungi, especially Alternaria, may play a role in pathogenesis of upper and lower airway diseases by activating PBMC. Allergic and non-allergic inflammatory reactions are involved in the pathogenesis of CRS.
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KEYWORD
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Fungus, Chronic sinusitis, Allergic rhinitis, Asthma, Peripheral blood mononuclear cell, Cytokine
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